Analysis Note

ControlMouse heart tissue lysate

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Western Blot Analysis: 1 µg/mL from a representative lot detected THAP11 on 10 µg of mouse liver tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected THAP11 in mouse cardiac muscle and mouse liver tissues.

This Anti-THAP11 (Ronin) Antibody is validated for use in Western Blotting, IHC for the detection of THAP11 (Ronin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

THAP11 (Ronin) is a transcriptional repressor which is predominantly expressed in embryonic stem (ES) cells. It contains a THAP-type zinc finger domain which is characteristic of the THAP 11 family members. In ES cells, THAP11 interacts with the transcriptional co-regulator HCF-1, to recruit chromatin-modifying proteins such as members of the polycomb family of proteins, to induce gene repression. THAP11 targets genes involved in metabolism, transcriptional initiation, and messenger RNA splicing. It plays an important role in maintaining the pluripotent state of ES cells.

Immunogen

KLH-conjugated linear peptide corresponding to human THAP11.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse heart tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected THAP11 on 10 µg of mouse heart tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~48 kDa observed. Uncharacterized bands appear at ~20-25 kDa in some lysates.